Optimizing production of Fc-amidated peptides by Chinese hamster ovary cells
نویسندگان
چکیده
منابع مشابه
Optimizing production of Fc-amidated peptides by Chinese hamster ovary cells
BACKGROUND Amidation of the carboxyl terminal of many peptides is essential for full biological potency, often increasing receptor binding and stability. The single enzyme responsible for this reaction is peptidylglycine α-amidating monooxygenase (PAM: EC 1.14.17.3), a copper- and ascorbate-dependent Type I membrane protein. METHODS To make large amounts of high molecular weight amidated prod...
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BACKGROUND The therapeutic use of α-amidated peptides (e.g. calcitonin, glucagon-like peptide) has increased dramatically, but there are major impediments to wider use of such peptides. Larger peptides are expensive to synthesize, and short plasma half-lives frequently limit the clinical circumstances in which the peptides would be useful. Both problems are potentially solved by producing pepti...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2015
ISSN: 1472-6750
DOI: 10.1186/s12896-015-0210-4